Cas9 Protein with NLS（Cas9 蛋白）
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Cas9 protein, in complex with guide RNA, offers benefit for CRISPR/Cas9 technology over plasmid or mRNA transfection. The biggest benefit is that you can make RNP complex that is ready to work in vivo. Cas9 protein is short lived (about 24 hours of half life in cells), thus minimize the off-target effects and mosaicism. You can also easily test the activity of guide RNA in vitro by a simple cleavage assay. In addition, Cas9 protein is pretty stable, and activity confirmation is much easier compared to mRNA. Lastly, it is active in more broad range of organisms such as nematodes, insects, plants, and non-typical model systems as it does not require transcription or translation.
Cas9 protein offered by PNA Bio is conjugated with NLS, and it is injection/transfection ready form.
Cy3-Cas9 can be an easy enrichment of the cells that are transfected with CRISPR/Cas9 complex.
